Β-methylamino-L-alanine (BMAA) was first implicated as an environmental factor responsible for the 50-100 times higher rate of motor neuron disease (MND) on the sland of Guam, in the 1950s. Since then, there has been increasing evidence for the presence of this neurotoxin in various aquatic and terrestrial environments and has been found to be produced by a variety of cyanobacteria and diatoms. The Riverina region, NSW, is considered an MND hotspot, and the frequent cyanobacterial blooms that occur within the Murrumbidgee catchment, have been suggested as the source for the seven times higher incidence of the disease. BMAA is usually found with two of its isomers, 2,4- diaminobutyric acid (2,4-DAB) and N-(2-aminoethyl) glycine (AEG) and has been shown to be co-produced by the same organisms that produce BMAA. LCMS/MS has proven to be the most sensitive and selective method for analysing BMAA, as it is able to differentiate BMAA from its isomers. In this study, cyanobacteria cells were isolated and cultured from samples collected in the Riverina region. The cultures were then analysed for BMAA, 2,4-DAB and AEG, using LC-MS/MS, to identify which species were capable of producing these compounds. The study also intends to determine whether growth phase and nitrate concentration, affect the production of BMAA and its isomers by BMAA producing cyanobacteria, by growing batch cultures in controlled laboratory conditions. The knowledge gained from this study help monitoring and managing the neurotoxin in the environment.