International research shows that urban land-use affects the distribution and population structure of odonates, but there are few Australian studies. Perth is a city of > 2 million people on the wetland-rich landscape of the Swan Coastal Plain. Perth displays varying degrees of urbanisation, from densely-developed new suburbs with little greenspace to less-dense older suburbs with larger vegetated areas, including around wetlands. This study compared the odonate assemblages of two groups of wetlands in these older suburbs with two groups of wetlands in a newer, more densely urbanised landscape. Surveys of larvae, adults and exuviae were carried out in spring 2019 and summer 2020 and water quality and habitat variables were measured at 22 wetlands. Exuviae of three common species were measured to determine size at emergence in response to wetland drying. Fifteen species from seven families were recorded breeding in the wetlands; adults only were recorded for 1 additional species. Assemblage composition did not differ, but regionally-endemic species were found only in wetlands in the less densely urbanised landscape. Higher cover and diversity of submerged and emergent vegetation were associated with higher larval diversity and abundance in spring; in summer, cooler water temperatures were also important. In spring, higher adult abundance and diversity were associated with higher water temperatures and emergent vegetation; in summer with terrestrial vegetation area only. Wetland area and distances between wetlands were not associated with any life stage. Assemblages of all life stages differed between sampling times due to species’ phenology and wetland drying. Hemicordulia tau, Orthetrum caledonicum and Xanthagrion erythroneurum emerged at smaller sizes in summer, accelerating growth to avoid desiccation in drying wetlands. Density of urban development did not influence odonate assemblage composition aside from two regionally-endemic species (Hesperocordulia berthoudi, Archiargiolestes pusillus) present in low numbers in the less densely urbanised landscape.